• Corpus ID: 199394248

Study of the disease detection for crop seedlings by using chlorophyll fluorescence

@inproceedings{Chiu2013StudyOT,
  title={Study of the disease detection for crop seedlings by using chlorophyll fluorescence},
  author={Yi Chich Chiu and Cheng-Kai Liao and Yung-Chiung Chang},
  year={2013}
}

• Y. Chiu, Cheng-Kai Liao, Yung-Chiung Chang
• Published 2013

This study used chlorophyll fluorescence image to detect and analyze the crop disease spots. The graphical programming language LabVIEW 2009 was used to develop the detection system, and Vision Assistant was used to construct image processing program for nondestructive detection of crop disease spots. The exciting light wavelength of the chlorophyll fluorescence used in this study was 430~470 nm. A blue ray LED was the light source, and a near infrared camera was used to capture 684±10 nm fluorescence released from crops. The crops were darkened, then the fluorescence images were taken and captured, and processed for disease spot recognition. This study used Dage pakchoi seedling and Fengxiang strawberry seedling as the crop subjects for test. The results showed that the disease spots on the leaves could be detected effectively by open, Gaussian Blur, exponential quantification, medium filtering, binarization and large segment removal image processing. The crops were treated by 20-min darkening in advance, and the disease spot recognition success rate could be reach over 80%. The crop leaves were sprayed with 400 ppm high dose pesticide to make pesticide spots, and the crops were placed in high temperature and high humidity stress at 35/28°C and 90% humidity to accelerate the formation of disease spots. The test results showed that the pesticide spot on the leaves of Dage pakchoi seedling was observed at the 22 hour after the spray of pesticide, and the pesticide spot area was 0.11mm. The pesticide spot on Fengxiang strawberry seedling was observed at the 16 hour, and the pesticide spot area was 0.50 mm. Over time, the pesticide spot area on Dage pakchoi seedling was 0.66 mm, and the pesticide spot area on Fengxiang strawberry seedling was 1.32 mm. The disease spot on Dage pakchoi seedling was observed at the 64 hour of high temperature and high humidity treatment, and the disease spot area was 0.11 mm. The disease spot on Fengxiang strawberry seedling was observed at the 52 hour, and the disease spot area was 0.11 mm. The disease spot area on Dage pakchoi seedling increased to 1.10 mm at the 96 hour, and that on Fengxiang strawberry seedling was 6 mm. A portable crop disease spot detection system may be developed from the results of this study in the future,