Study of rust resistance genes in wheat germplasm with DNA markers

Abstract

Wheat is a vital dietary component for human health and widely consumed in the world. Wheat rusts are dangerous pathogens and contribute serious threat to its production. In present study, PCR-Based DNA Markers were employed to check the rust resistance genes among 20 wheat genotypes and 22 markers were amplified. NTSYS-pc 2.2 was used to calculate genetic diversity and Nei and Li's coefficients ranged from 0.55 to 0.95. Cluster analysis was obtained using UPGMA (Unweighted Pair Group Method of Arithmetic Average) algorithm. Maximum no. of genes (23) was amplified from TW-760010 genotype whereas minimum no of genes (14) were amplified from TW-76005 genotype. The data gained from present study open up new ways to produce new varieties by breeding rust resistant germplasm to avoid the economic and food loss and varieties with improved characteristics.

DOI: 10.6026/97320630010371

Extracted Key Phrases

8 Figures and Tables

Cite this paper

@inproceedings{Haque2014StudyOR, title={Study of rust resistance genes in wheat germplasm with DNA markers}, author={Asma Haque and Tayyaba Shaheen and Tahsin Gulzar and Mahmood ur Rahman and Fatima Jalal and Summera Sattar and Beenish Ehsan and Zafar Iqbal and Muhammad Younas}, booktitle={Bioinformation}, year={2014} }