Study of rumen metagenome community using qPCR under different diets

@article{Singh2014StudyOR,
  title={Study of rumen metagenome community using qPCR under different diets},
  author={K. M. Singh and P. Pandya and A. Tripathi and G. Patel and S. Parnerkar and R. Kothari and C. Joshi},
  journal={Meta Gene},
  year={2014},
  volume={2},
  pages={191 - 199}
}
The aim of this study was to detect the major bacteria present in rumen microbiota. Here, we performed qPCR based absolute quantitation of selected rumen microbes in rumen fluid of river buffalo adapted to varying proportion of concentrate to roughage diets. Animals were adapted to roughage-to-concentrate ratio in the proportion of 100:00 (T1), 75:25 (T2), 50:50 (T3) and 25:75 (T4) respectively for 30 days. At the end of each treatment, rumen fluid was collected at 0 h and 2 h after feeding. It… Expand
Whole rumen metagenome sequencing allows classifying and predicting feed efficiency and intake levels in cattle
TLDR
The findings indicated that there are differences between the microbiota compositions of high and low-efficiency animals both at the taxonomical and gene levels, and even more evident in terms of intake levels. Expand
Effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle
TLDR
Providing sugarcane with high NDFD favored the growth of fibrolytic bacteria, and this effect were dependent on the conservation method and on diet concentrate level, while sucrose appears to have great effect on the composition of ruminal microflora, especially S. bovis. Expand
Metagenomic Approaches in Understanding the Rumen Function and Establishing the Rumen Microbial Diversity
TLDR
Applied metagenomics has the potential for providing insight into the functional dynamics of the ruminomics database and will help to achieve a major goal of rumen ecosystem; microbial communities function and interact among these microbes as well as with the host. Expand
Effect of different types of olive oil pomace dietary supplementation on the rumen microbial community profile in Comisana ewes
Olive oil pomace (OOP) is a bio-waste rich in highly soluble polyphenols. OOP has been proposed as an additive in ruminant feeding to modulate rumen fermentations. Three groups of ewes were fed theExpand
Identification and Genomic Characterization of Candidate Starch and Lactate Utilizing Bacteria from the Rumen of Beef Cattle
TLDR
The main objective of the research described in this dissertation was to identify previously uncharacterized bacteria based on their ability to grow in the presence of starch or lactate using an in-vitro batch culture approach. Expand
Survival of Lactobacillus plantarum U 40 on the in vitro rumen fermentation quantified with real-time PCR
The objective of this study was to evaluate the survival of L. plantarumU40 quantified with realtime PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block designExpand
Microbial populations and ruminal fermentation of sheep and llamas fed low quality forages
TLDR
The llama forestomach content showed higher degree of fermentative activity than the sheep one, as indicated by NH3-N and VFA values, and the forestomach of llamas contained higher density of total protozoa and Eudiplodinium. Expand
Links between the rumen microbiota, methane emissions and feed efficiency of finishing steers offered dietary lipid and nitrate supplementation
TLDR
The effects of dietary additives designed to reduce methane emissions on the rumen microbiota, and relationships with feed efficiency within dietary treatment groups are explored, are assessed. Expand
Isolation of chitinolytic Clostridium sp. NCR from Mehsani buffalo rumen, its genomic analysis and potential role in rumen
Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tagsExpand
S0021859619000534jra 357..364
Intake in sugar-rich diets can be limited either via rumen fill or excessive rumen fermentation and source of non-fibre carbohydrate (NFC) in the diet can affect both factors. The aim of the currentExpand
...
1
2
3
...

References

SHOWING 1-10 OF 46 REFERENCES
Use of Real-Time PCR Technique in Studying Rumen Cellulolytic Bacteria Population as Affected by Level of Roughage in Swamp Buffalo
TLDR
It was found that feeding urea-treated rice straw solely increased these three cellulolytic bacteria numbers up to 2.65 × 109 and 3.54 ×-109 copies per milliliter for F. succinogenes, R. flavefaciens, and R. albus in rumen fluid and digesta, respectively. Expand
Inclusion of novel bacteria in rumen microbiology: Need for basic and applied science
TLDR
Specific uncultured bacterial groups are being considered as important members of a fibrolytic consortium in the rumen, judging by their ecologic distribution and could potentially be candidates as probiotics and sources of enzymes for animal feed and other industrial uses. Expand
Real-Time PCR Detection of the Effects of Protozoa on Rumen Bacteria in Cattle
TLDR
It was clarified here that an absence of protozoa exerted differential effects on the populations of cellulolytic bacteria in cattle rumens (i.e., F. succinogenes, R. albus, and R. flavefaciens), and real-time PCR analysis suggested that the CUR-E cluster was more prevalent in the unfaunated rumens. Expand
The rumen bacteria
TLDR
It is to be hoped that the major obstacles to cultivation of the most numerous rumen bacteria have been overcome by the development of sufficiently rigorous anaerobic methods and of suitable isolation media. Expand
Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens.
TLDR
Competitive PCR assays were developed for the enumeration of the rumen cellulolytic bacterial species: Fibrobacter succinogenes, R. albus and R. flavefaciens and the population size of the three species did not change after the proportion of dietary alfalfa hay was increased. Expand
Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases
TLDR
Comparison of the glycoside hydrolase and cellulosome functional genes revealed that in the rumen microbiome, initial colonization of fiber appears to be by organisms possessing enzymes that attack the easily available side chains of complex plant polysaccharides and not the more recalcitrant main chains, especially cellulose. Expand
A comparison of enzymatic and molecular approaches to characterize the cellulolytic microbial ecosystems of the rumen and the cecum.
TLDR
The cellulolytic bacterial community structure was different in the two compartments, with F. succinogenes as the main species in the rumen and R. flavefaciens in the cecum, and other hypotheses related to digestion are proposed. Expand
Kinetics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR.
TLDR
F. succinogenes was most dominant, both in the whole rumen digesta and on the suspended hay stems, demonstrating the ecological and functional significance of this species in ruminal fiber digestion. Expand
Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR.
TLDR
Jejunum digesta samples were taken from weaning pigs in order to evaluate real-time PCR (qPCR) as a method for quantifying pig gut bacteria and values obtained by qPCR and traditional methods showed a significant correlation for lactobacilli and total bacteria. Expand
How many ruminal bacteria are there?
TLDR
Studies of 16S rRNA and DNA:DNA hybridization indicate that the diversity of ruminal bacteria has been greatly underestimated, and the DNA: DNA hybridization is a more sensitive method of assessing bacterial relatedness than is 16S sRNA. Expand
...
1
2
3
4
5
...