Study of hydrophobic interaction adsorption of bovine serum albumin under overloaded conditions using flow microcalorimetry.


The adsorption behavior of bovine serum albumin (BSA) on a Sepharose based hydrophobic interaction support has been studied. Flow microcalorimetry has been used to determine the heat of adsorption under overloaded chromatographic conditions. These data have been complemented with capacity factor and isotherm measurements to provide insight on the mechanisms… (More)


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