Study of heme Fe(III) ligated by OH- in cytochrome b-559 and its low temperature photochemistry in intact chloroplasts.

@article{Fiege1995StudyOH,
  title={Study of heme Fe(III) ligated by OH- in cytochrome b-559 and its low temperature photochemistry in intact chloroplasts.},
  author={Robert Fiege and Ulrich Schreiber and G. Renger and Wolfgang Lubitz and Vladimir A. Shuvalov},
  journal={FEBS letters},
  year={1995},
  volume={377 3},
  pages={325-9}
}
EPR properties of Cyt b-559 have been investigated in intact chloroplasts that are functionally competent in O2 evolution and in CO2 fixation. After chemical oxidation of Cyt b-559 by 10 mM 2,3-dicyano, 4,5-dichloro-p-benzoquinone (DDQ) the major part of Cyt b-559 is found to be present in the high spin Fe(III) form. Only a small fraction of low spin heme Fe(III) (less than 5%) was formed by chemical or light-induced oxidation. This fraction increased during aging of intact chloroplasts. A… CONTINUE READING

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EPR properties of Cyt b-559 have been investigated in intact chloroplasts that are functionally competent in O2 evolution and in CO2 fixation .
EPR properties of Cyt b-559 have been investigated in intact chloroplasts that are functionally competent in O2 evolution and in CO2 fixation .
IronNo subtypeFerric
When in intact chloroplasts the heme Fe is chemically oxidized to Fe(III ) ligated by OH- , this OH- ligation disappeared after a subsequent illumination at 80 K by red light .
A comparison with the EPR signal of Fe(III ) in myoglobin ( Mb ) reveals that the structure of the high spin signal in intact chloroplasts is indicative for the presence of an axial OH- ligand at the heme Fe(III ) .
FerricNo subtypeIron
A comparison with the EPR signal of Fe(III ) in myoglobin ( Mb ) reveals that the structure of the high spin signal in intact chloroplasts is indicative for the presence of an axial OH- ligand at the heme Fe(III ) .
When in intact chloroplasts the heme Fe is chemically oxidized to Fe(III ) ligated by OH- , this OH- ligation disappeared after a subsequent illumination at 80 K by red light .
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