Study of functional interaction between three copies of the insulator from the MDG4 transposable element in the model system of the miniwhite gene of Drosophila melanogaster

Abstract

239 Insulators are regulatory DNA elements blocking the interaction between enhancer and promoter when interposed between them [1]. Insulators do not inactivate enhancer and promoter directly. It was shown that the enhancer that is separated from one promoter by an insulator is still able to activate another, unseparated promoter [1]. The Drosophila melanogaster insulator found in the 5'-regulatory region of the MDG4 retrotransposon ( gypsy ) has been studied most comprehensively [2]. This insulator is a 430-bp DNA fragment comprising 12 copies of the consensus sequence 5'-PyPuTTGCATACCPy-3', to which the protein coded by the su(Hw) gene binds [3]. Inactivation of this gene results in the loss of insulation and restoration of enhancer–promoter interactions.

DOI: 10.1134/S1607672908040194

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Cite this paper

@article{Kostyuchenko2008StudyOF, title={Study of functional interaction between three copies of the insulator from the MDG4 transposable element in the model system of the miniwhite gene of Drosophila melanogaster}, author={Margarita V Kostyuchenko and Ekaterina Savitskaya and I. A. Volkov and Anton Golovnin and Pavel Georgiev}, journal={Doklady Biochemistry and Biophysics}, year={2008}, volume={421}, pages={239-243} }