Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line

@article{Cook2008StudyOA,
  title={Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line},
  author={Brian L. Cook and Karin E. Ernberg and Hyeyoun Chung and Shuguang Zhang},
  journal={PLoS ONE},
  year={2008},
  volume={3},
  pages={1029 - 1038}
}
In order to begin to study the structural and functional mechanisms of olfactory receptors, methods for milligram-scale purification are required. Here we demonstrate the production and expression of a synthetically engineered human olfactory receptor hOR17-4 gene in a stable tetracycline-inducible mammalian cell line (HEK293S). The olfactory receptor gene was fabricated from scratch using PCR-based gene-assembly, which facilitated codon optimization and attachment of a 9-residue bovine… CONTINUE READING