Study of Heterogeneous Emission of Parinaric Acid Isomers Using Multifrequency Phase Fluorometry

  title={Study of Heterogeneous Emission of Parinaric Acid Isomers Using Multifrequency Phase Fluorometry},
  author={T. Parasassi and F. Conti and E. Gratton},
Author(s): Parasassi, T; Conti, F; Gratton, E | Abstract: The fluorescence lifetimes of parinaric acid (PnA) isomers have been measured in pure solvents and in synthetic phospholipid membranes over a wide temperature range. In pure solvents, dodecane, ethanol, and cyclohexanol, the emission is well described by three exponential components of approximately 40, 12, and 2 ns. The preexponential factor of the long-lifetime component is quite small, and this component is neglected in the present… Expand
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Data are presented for both parinaric acid isomers in dimyristoylphosphatidylcholine membranes collected to higher precision, and the fluorescence decays are shown to be best described by a smooth distribution of decay times rather than by a few discrete lifetimes. Expand
Title Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence Permalink
The sensitivity of Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) excitation and emission spectra to the physical state of the membrane arises from dipolar relaxation processes in the membraneExpand
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Lateral heterogeneity in human platelet plasma membrane and lipids from the time-resolved fluorescence of trans-parinaric acid
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The determined dynamic properties of the hydrocarbon region in the lecithin liposomes differ markedly from those of the sphingomyelin liposome, which indicates that the forces which dictate the dynamic properties in the hydro carbon region in lipid bilayers predominantly originate from hydrophobic interactions. Expand
The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
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Fluorescence depolarization in liquid crystals and membrane bilayers
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