Studies pertaining to the purification and properties of thymine 7-hydroxylase.

  title={Studies pertaining to the purification and properties of thymine 7-hydroxylase.},
  author={Ralph P. Mccroskey and Wait R. Griswold and R. L. Sokoloff and E. Dale Sevier and S. H. Lin and K Liu and Patricia M. Shaffer and R D Palmatier and Thomas S. Parker and Mitchel T. Abbott},
  journal={Biochimica et biophysica acta},
  volume={227 2},
Metabolism of pyrimidine deoxyribonucleosides in Neurospora crassa
The experiments in this report involve the following series of reactions which were previously demonstrated with purified enzyme preparations from Neurospora crassa: thymidine a yields thymine
Regulation of thymidine metabolism in Neurospora crassa
It is suggested that the 2'-hydroxylase has, in addition to the role of salvaging the pyrimidine ring, therole of providing ribose not only for the utilization of the demethylated pyridine but also for other metabolic processes.
Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.
Cloning and characterization of Rhodotorula glutinis thymine hydroxylase.
The cloned enzyme characterized in this report displayed substantial activity, indicating that the sequence differences are critical for activity, and the substrate selectivity of TH against a series of pyrimidine analogues is consistent with that reported for the wild-type enzyme and, in part, explains the mode of selection of uracil analogues.
A non-heme iron protein with heme tendencies: an investigation of the substrate specificity of thymine hydroxylase.
Thymine hydroxylase from Rhodotorula glutinis catalyzes the oxidation of thymine to its alcohol, aldehyde, and carboxylic acid in three successive reactions. Each step involves stoichiometric


Mutants Affecting Thymidine Metabolism in Neurospora crassa
Three mutants were isolated in which different steps are blocked in the pathway that converts the pyrimidine ring of thymidine to an RNA precursor, and three other classes of mutations are proposed to be affecting, respectively, regulation of theThymidine degradative pathway, transport of pyridine free bases, and transport ofpyrimidine nucleosides.
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on