Studies on the use of a novel affinity matrix, sepharose amine-succinyl-amine haloperidol hemisuccinate, ASA-HHS, for purification of canine dopamine (D2) receptor.

@article{Antonian1986StudiesOT,
  title={Studies on the use of a novel affinity matrix, sepharose amine-succinyl-amine haloperidol hemisuccinate, ASA-HHS, for purification of canine dopamine (D2) receptor.},
  author={Lida Antonian and Edna Antonian and Randall B. Murphy and David I Schuster},
  journal={Life sciences},
  year={1986},
  volume={38 20},
  pages={
          1847-58
        }
}
12 Citations
Purification of the D-2 dopamine receptor from bovine striatum.
Characterization of binding sites for [3H]spiroperidol.
Effect of AL 721, a Novel Membrane Fluidizer, on the Binding Parameters of Brain Dopamine Receptors
TLDR
The results suggest that removal of the natural lipids during purification diminishes the binding ability of the D2 receptor and that direct in vitro modulation of rat striatal membrane fluidity is effective in restoring the affinity of dopamine receptors, which have diminished upon aging.
Discussion of the Paper
TLDR
A novel affinity matrix, sepharose amine-succinyl-amine haloperidol hemisuccinate, is used for purification of canine dopamine (D2) receptor through Affinity chromatography of the anterior pituitary D2-dopamine receptor.
Biochemical Characterization of Dopamine Receptors
Following the recognition of dopamine as a neurotransmitter in the brain, independent of its role as a precursor of noradrenaline, there has been great interest in the sites of action of dopamine,
Phosphorylation by Cyclic AMP‐Dependent Protein Kinase Modulates Agonist Binding to the D2 Dopamine Receptor
Abstract: Phosphorylation of striatal membranes by cyclic AMP‐dependent protein kinase resulted in a reduction in the affinity of the D2 dopamine receptor toward its agonist N‐propylnorapomorphine
Dopamine receptors and the dopamine hypothesis of schizophrenia
TLDR
Direct evidence that neuroleptics selectively blocked dopamine receptors occurred in 1974 with the finding that nanomolar concentrations of these drugs stereoselectively inhibited the binding of [3H]‐dopamine or [3h]‐haloperidol.
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References

SHOWING 1-10 OF 25 REFERENCES
Assay for soluble dopamine receptors by the precipitation method.
Solubilisation of high-affinity dopamine receptors
TLDR
The solubilisation of binding sites from dog striatum are reported which retain the characteristics of membrane-bound dopamine receptors.
Drug Potencies on Partially Purified Brain D2 Dopamine Receptors
TLDR
The absence of receptors in the pH 6.8 and pH 10 fractions, although both contained prelabeled [3H]spiperone, indicates the importance of testing agonists and antagonists on each fraction at each step in purification.
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
  • P. Cuatrecasas
  • Biology, Chemistry
    The Journal of biological chemistry
  • 1970
TLDR
It is demonstrated that successful application of affinity chromatography in many cases will critically depend on placing the ligand at a considerable distance from the matrix backbone.
The characterization of actin associated with postsynaptic membranes from Torpedo californica.
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