Studies on the subcellular mechanisms mediating the negative estradiol effect on GnRH-induced LH-release by rat pituitary cells in culture.

@article{Emons1989StudiesOT,
  title={Studies on the subcellular mechanisms mediating the negative estradiol effect on GnRH-induced LH-release by rat pituitary cells in culture.},
  author={Guenter Emons and Ernst Uli Frevert and Olaf Ortmann and U Fingscheidt and Rita Sturm and Ludwig Kiesel and Rudolf Knuppen},
  journal={Acta endocrinologica},
  year={1989},
  volume={121 3},
  pages={350-60}
}
Cultured pituitary cells from adult female rats were treated for 4 or 24 h in the absence or presence of E2 (10(-9) mol/l) with increasing concentrations of the transcription inhibitor actinomycin-D or the translation inhibitor puromycin. During the last 4 h of incubation, LH release was stimulated with 5 X 10(-10) mol/l GnRH. The positive E2 effect… CONTINUE READING