Studies on the structure of the bioregulator purified from the rat brain

Abstract

From the brain tissue of Wistar rats, we purified a bioregulator, which is active at ultralow doses. Using reversed-phase HPLC, we prepared a homogenous polypeptide with a molecular weight of 4749 ± 2 Da, which is responsible for the biological activity of the bioregulator. Using the CD spectroscopy method, we calculated the percentage of canonical elements of the secondary polypeptide structure in a solution. Using the methods of proteomics, we revealed that the structure of the investigated polypeptide was similar to the N-terminal sequence of a fragment of guanine-nucleotide binding G0-protein subunit α-1.

DOI: 10.1134/S0003683814030077

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@article{Ilina2014StudiesOT, title={Studies on the structure of the bioregulator purified from the rat brain}, author={A. P. Il’ina and Anton Molyavka and V. P. Yamskova and Aleksei K Buryak and Igor A Yamskov}, journal={Applied Biochemistry and Microbiology}, year={2014}, volume={50}, pages={398-403} }