• Corpus ID: 1160839

Studies on the rate of reduction of hepatic microsomal cytochrome P-450 by reduced nicotinamide adenine dinucleotide phosphate: effect of drug substrates.

@article{Gigon1969StudiesOT,
  title={Studies on the rate of reduction of hepatic microsomal cytochrome P-450 by reduced nicotinamide adenine dinucleotide phosphate: effect of drug substrates.},
  author={Philippe Gigon and Theodore E. Gram and James R. Gillette},
  journal={Molecular pharmacology},
  year={1969},
  volume={5 2},
  pages={
          109-22
        }
}
Optimum conditions are described for the assay of cytochrome P-450 reduction by NADPH in rat liver microsomes. The addition in vitro of chemical compounds which produce type I spectral changes markedly accelerated the initial rate of NADPH-linked cytochrome P-450 reduction, while the addition of compounds which elicit type II spectral changes significantly decelerated the rate. These changes occurred in the absence of appreciable changes in the absorbance of the cytochrome P-450—CO complex… 
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