Studies on the primary sequence requirements for PKC-alpha, -beta 1 and -gamma peptide substrates.

@article{Marais1990StudiesOT,
  title={Studies on the primary sequence requirements for PKC-alpha, -beta 1 and -gamma peptide substrates.},
  author={Richard Marais and Oliver F Nguyen and James R Woodgett and Peter J Parker},
  journal={FEBS letters},
  year={1990},
  volume={277 1-2},
  pages={151-5}
}
The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguishable in their phosphorylation of these peptides, PKC-gamma shows a distinct specificity profile for these synthetic substrates.