Studies on the methyl isocyanate adducts with globin.

@article{Mrz2004StudiesOT,
  title={Studies on the methyl isocyanate adducts with globin.},
  author={J. Mr{\'a}z and P. {\vS}imek and D. Chvalov{\'a} and H. Nohov{\'a} and P. {\vS}migolov{\'a}},
  journal={Chemico-biological interactions},
  year={2004},
  volume={148 1-2},
  pages={
          1-10
        }
}
Isocyanates such as methylisocyanate (MIC), an intermediate in the synthesis of carbamate pesticides, or diisocyanates, used in the production of plastics, are highly reactive toxic compounds that spontaneously bind to biological macromolecules. In vivo formation of stable adducts with blood protein globin offers possibilities for biomonitoring of internal exposure to various reactive species. Thus, biomonitoring of the isocyanates through determination of their specific adducts with globin is… Expand
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TLDR
It is concluded that conjugation of methyl isocyanate with glutathione in vivo affords a reactive S-linked product which displays the potential to carbamoylate nucleophilic amino acids. Expand
N-methylcarbamoyl adducts at the N-terminal valine of globin in workers exposed to N,N-dimethylformamide
TLDR
The work indicates the formation of MIC during the metabolism of DMF as identical adducts were also found after exposure to methylisocyanate (MIC). Expand
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Convugation of MIC with glutathione (GSH), followed by metabolism of the resulting adduct to AMCC appears to represent a quantitatively important pathway of biotransformation of MIC in the rat. Expand
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N-terminal amino acids and Lys residues are likely to produce most abundant adducts with diisocyanates in proteins, which reflects the mechanism of the amine- isocyanate reaction. Expand
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TLDR
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TLDR
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Toxicokinetic studies in volunteers laid the grounds for setting the reference value for biological monitoring of occupational exposure to DMF, and has been used to determine the background levels of MVH in unexposed subjects. Expand
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