Studies on the mechanism and kinetics of the 2-oxoglutarate dehydrogenase system from pig heart.

@article{McMinn1977StudiesOT,
  title={Studies on the mechanism and kinetics of the 2-oxoglutarate dehydrogenase system from pig heart.},
  author={Charles McMinn and J. H. Ottaway},
  journal={The Biochemical journal},
  year={1977},
  volume={161 3},
  pages={569-81}
}
1. The kinetic properties of the 2-oxoglutarate dehydrogenase system were investigated. To this end, initial-velocity studies were carried out by the method of Fromm [(1967) Biochim. Biophys. Acta 139, 221-230]. Reciprocal plots of the results did not agree with those expected for the Hexa Uni Ping Pong mechanism previously proposed for the system. 2. The measured initial velocities were fitted to initial-rate equations corresponding to several possible mechanisms by using a computer… CONTINUE READING