Studies on the enzymology of purified preparations of brush border from rabbit kidney.

@article{George1973StudiesOT,
  title={Studies on the enzymology of purified preparations of brush border from rabbit kidney.},
  author={Stephen G. George and John D. Kenny},
  journal={The Biochemical journal},
  year={1973},
  volume={134 1},
  pages={
          43-57
        }
}
1. A method for the preparation of brush border from rabbit kidneys is described. Contamination by other organelles was checked by electron microscopy and by the assay of marker enzymes and was low. 2. Seven enzymes, all hydrolases, were substantially enriched in the brush-border preparation and are considered to be primarily located in this structure. They are: alkaline phosphatase, maltase, trehalase, aminopeptidase A, aminopeptidase M, gamma-glutamyl transpeptidase and a neutral peptidase… CONTINUE READING

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