Studies on the effect of tetrandrine on microtubules. I. Biochemical observation and electron microscopy.

@article{Liu1988StudiesOT,
  title={Studies on the effect of tetrandrine on microtubules. I. Biochemical observation and electron microscopy.},
  author={Leroy Fong Liu and Natasha Chen and Guo Ping Cai and Zi Ling Li and Janet G. Yang and Yan Rong Li},
  journal={Ecotoxicology and environmental safety},
  year={1988},
  volume={15 2},
  pages={
          142-8
        }
}
Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirect immunofluorescent method including tubulin antibody to locate the presence of microtubules in 3T3 cells by fluorescence microscopy. The effects of colchicine and P204 were studied for comparison at… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.