Studies on the chemical nature of the substance inducing transformation of pneumococcal types; effect of desoxyribonuclease on the biological activity of the transforming substance.

@article{Mccarty1946StudiesOT,
  title={Studies on the chemical nature of the substance inducing transformation of pneumococcal types; effect of desoxyribonuclease on the biological activity of the transforming substance.},
  author={Maclyn Mccarty and Oswald T. Avery},
  journal={The Journal of experimental medicine},
  year={1946},
  volume={83},
  pages={
          89-96
        }
}
It has been shown that extremely minute amounts of purified preparations of desoxyribonuclease are capable of bringing about the complete and irreversible inactivation of the transforming substance of Pneumococcus Type III. The significance of the effect of the enzyme, and its bearing on the chemical nature of the transforming substance, together with certain considerations concerning the biological specificity of desoxyribonucleic acids in general, are discussed. 
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109 Citations
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109 Citations

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Citation Type

Purification of the desoxypentose nucleic acid of Hemophilus influenzae having transforming activity.
CHEMICAL NATURE AND BIOLOGICAL SPECIFICITY OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES
TLDR
The present work provides another example of the potential relationship of the knowledge gained from microbiological investigations to problems in other fields of scientific research.
Studies on the resistance of desoxyribonucleic acids to physical and chemical factors.
BACTERIAL TRANSFORMATION REACTIONS
TLDR
The principles involved and the results observed in the study of this reaction have implications beyond those referable to the biology of pneumococcus and are correlated with earlier work concerned with bacterial transformations.
Studies on the desoxypentose nuclease of yeast and its specific cellular regulation.
Milestones of studying the mechanisms of the genetic bacterial transformation on the model of Streptococcus
The chronology for the main stages of studying bacterial genetic transformation is presented. Streptococci (pneumococci in particular) were used as the model organisms. Recent data on the mechanisms…
Contribution on the biochemical investigation of external factors required for type transformation in thePneumococcus
TLDR
It was found that different anti-R-Pneumococcus sera display a great variability in DNase activity contained therein, and the presence of suitable concentrations of salts with univalent cations in the transformation medium inhibits theDNase activity in it, thus regularizing the results obtained during type transformation in the Pneumitis.
Multiple forms of deoxyribonuclease I
  • T. Liao
  • Biology
    Molecular and Cellular Biochemistry
  • 2006
TLDR
The chemical basis of the multiple forms in bovine pancreas will be discussed in detail, and the evidence for multiplicity will be presented for other DNases, including those in ovine Pancreas, bovines, mouse and rat parotid, and malt.
Traditional and molecular analyses of the genes encoding pneumococcal polysaccharide capsule production: cloning Avery's transforming principle
TLDR
Following earlier work on transformation by Fred Griffith and others, Oswald Avery and colleagues showed that DNA, and not some other cellular component was responsible for the transformation of pneumococci from an unencapsulated to an encapsulated state.
The discovery of pneumococcal type transformation: an appreciation
  • W. Hayes
  • Biology, Medicine
    Journal of Hygiene
  • 1966
TLDR
A series of careful and painstaking experiments show beyond doubt that the ability to produce a polysaccharide capsule can regularly be restored to ‘rough’ (R) strains of pneumococci which have lost it, by the subcutaneous inoculation of mice with an excess of heat-killed, capsulated (S) virulent bacteria.
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