Studies on the amino groups of myosin ATPase. IV. Effects of ATP and its analogs on the spectral properties of trinitrophenylated myosin and its active fragments.

A considerable blue shift was observed in the absorption spectrum of the trinitrophenyl moiety attached to a functional epsilon-lysyl amino group of subfragment-1, heavy meromyosin and myosin on addition of ATP or ATP analogs. The resulting difference spectra showed a maximum at 320 and a minimum at 365 nm. The greatest spectral change was observed with a… CONTINUE READING