Studies on the Steroid Hydroxylation System in Adrenal Cortex Microsomes

@inproceedings{Kominami2001StudiesOT,
  title={Studies on the Steroid Hydroxylation System in Adrenal Cortex Microsomes},
  author={Shiro A J Kominami and Hiroshi Ochi and Yuhko Kobayashi and Shigeki Takemori},
  year={2001}
}
Cytochrome P-450 was purified to a specific content of 16 nmol/mg of protein from bovine adrenal cortex microsomes by hydrophobic chromatography using an w-amino-n-octyl Sepharose column in the presence of Emulgen 913, a nonionic detergent. The purified preparation exhibited a single polypeptide band (Mr = 47,000 f 1,000) when submitted to sodium dodecyl… CONTINUE READING