Studies on proteinases from the digestive organs of sardine. II. Purification and characterization of two acid proteinases from the stomach.

@article{Noda1981StudiesOP,
  title={Studies on proteinases from the digestive organs of sardine. II. Purification and characterization of two acid proteinases from the stomach.},
  author={Masatoshi Noda and Kazuo Murakami},
  journal={Biochimica et biophysica acta},
  year={1981},
  volume={658 1},
  pages={27-34}
}
Two fish acid proteinases designated acid proteinase I and II were found and isolated by (NH4)2SO4 fractionation, CM-cellulose chromatography and gel filtration on Sephadex G-100. The final preparations were judged nearly homogeneous by multiple criteria. The molecular criteria. The molecular weights of the enzymes I and II were determined by the sedimentation equilibrium method to be 37 000 and 33 400, respectively. The sedimentation coefficients (S0 20, w) were 3.06 and 3.09, respectively… CONTINUE READING
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