Studies on plasma membranes. IV. The ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma.
@article{Benedetti1967StudiesOP, title={Studies on plasma membranes. IV. The ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma.}, author={E. Benedetti and P. Emmelot}, journal={Journal of cell science}, year={1967}, volume={2 4}, pages={ 499-512 } }
Plasma membranes were isolated from rat liver and a transplanted rat hepatoma of the hepatocellular type. After glutaraldehyde fixation the membranes were treated with colloidal iron hydroxide (CIH) at pH 1.7, which was found to react specifically with the neuraminidase-sensitive sialic acid of the liver membranes. The CIH-reactive, neuraminidase-sensitive sialic acid, comprising 70% of the membrane-bound sialic acid, was exclusively located in the outer leaflet of the liver membranes as shown… Expand
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