Studies on plant regeneration from protoplasts in the genus Helianthus

@article{Chanabe1991StudiesOP,
  title={Studies on plant regeneration from protoplasts in the genus Helianthus},
  author={Christel Chanabe and Monique Burrus and Dennis Bidney and Gilbert Alibert},
  journal={Plant Cell Reports},
  year={1991},
  volume={9},
  pages={635-638}
}
Protoplasts were produced from 7-day-old hypocotyls of two cultivated sunflower genotypes and three wild sunflowers. When included in agarose droplets and cultured in TL medium supplemented with 0.1 mg/l 2,4-dichlorophenoxyacetic acid, the protoplasts gave rise to loose colonies and to “embryoids”. After two months the small calli emerging from the agarose were transferred to a regeneration medium on which they grew and began to differentiate. A second transfer to the same medium 40 days later… CONTINUE READING

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