Studies on lung N-methyltransferases, a pharmacological approach

  title={Studies on lung N-methyltransferases, a pharmacological approach},
  author={Robert G. Pendleton and George W. Gessner and John L. Sawyer},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
SummaryPhenylethanolamine N-methyltransferase (PNMT) was identified as the primary, high affinity N-methylating enzyme for phenylethanolamine (PEA) in rat, dog and Rhesus monkey lung. Human and rabbit lung, however, do not contain this enzyme but possess a more non-specific or general N-methyltransferase with a relatively low affinity for PEA and for which β-phenethylamine (β-PE) is also a substrate. The former but not the latter enzyme is markedly inhibited by micromolar concentrations of the… Expand
2 Citations
Trace Amines: An Overview
Two TAs act as regular transmitters in invertebrates where they take the roles of epinephrine and norepinephrine that are absent in these organisms, making them valid targets for specific insecticides. Expand
The emerging roles of human trace amines and human trace amine-associated receptors (hTAARs) in central nervous system.
Findings are discussed that provide insights into the functional roles of human trace amines (including P-Octopamine, β phenylethylamine, Tryptamine, Tyramine, Synephrine, 3-Iodothyronamine, N-Methyltyramine) in brain and the known functions of human Trace amine receptors in brain. Expand


Studies on adrenal phenylethanolamine N- methyltransferase (PNMT) with S K & F 64139, a selective inhibitor.
Evidence for in vivo phenylethanolamine N-methyltransferase inhibitory activity was that the drug markedly inhibited the conversion of a tracer dose of 3H-norepinephrine to3H-epinephrine in the rat adrenal gland after unit oral doses as low as 5 mg/kg. Expand
Studies on the characterization and inhibition of rat brain phenylethanolamine N-methyltransferase
SummaryThe existence of phenylethanolamine N-methyltransferase (PNMT) activity in the rat brain and spinal cord was confirmed using both substrate specificity and selective inhibitors of the adrenalExpand
Studies on the distribution of phenylethanolamine N-methyltransferase and epinephrine in the rat.
The data indicate that although the adrenal is the principal tissue source of epinephrine and PNMT in the rat, small amounts of both substances are present in several body organs. Expand
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The serotonin methylating enzyme can N-methylate a variety of normally occurring compounds such as dopamine, normetanephrine, metanephine, epinephrine, tyramine, and octopamine as well as a varietyof foreign compounds suchAs ephedrine, amphetamine, aniline, normorphine,normeperidine and nornicotine. Expand
Inhibition of transmethylations of biogenic amines by S-adenosylhomocysteine. Enhancement of transmethylation by adenosylhomocysteinase.
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Protein measurement with the Folin phenol reagent.
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein. Expand
Statistical Methods
P > 0.05 0.01 < P < 0.05 0.001 < P < 0.01 * * * multiple regression analyses were tested by t tests. Regression lines were compared by means of analysis of covariance in which differences in slopesExpand
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