Studies on intracellular translocation of apolipoprotein B in a permeabilized HepG2 system.

@article{Macri1997StudiesOI,
  title={Studies on intracellular translocation of apolipoprotein B in a permeabilized HepG2 system.},
  author={Joseph Macri and Khosrow Adeli},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 11},
  pages={7328-37}
}
Recent evidence suggests that the rate of apolipoprotein B-100 (apoB) translocation may be a key regulatory point in the production of apoB-containing lipoproteins. We have developed an in vitro system to measure the translocation rate of apoB in HepG2 cells. Intact cells were initially pretreated with oleate and N-acetyl-Leu-Leu-norleucinal to maximize the translocation rate while minimizing degradation. Cells were pulsed with [35S]methionine, chased (5-30 min), and then permeabilized with… CONTINUE READING

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