Studies on interaction between Vitamin B12 and human serum albumin.


The binding reaction between Vitamin B12 (B12, cyanocobalamin) and human serum albumin (HSA) was investigated by fluorescence quenching, UV-vis absorption and circular dichroism (CD) spectroscopy. Under simulative physiological conditions, fluorescence quenching data revealed that the quenching constants (Ksv) are 3.99 x 10(4), 4.33 x 10(4), 4.76 x 10(4… (More)
DOI: 10.1016/j.jpba.2007.12.029

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