Studies on depth-of-field effects in microscopy supported by numerical simulations.

@article{Overby2005StudiesOD,
  title={Studies on depth-of-field effects in microscopy supported by numerical simulations.},
  author={Darryl R. Overby and Mark W. Johnson},
  journal={Journal of microscopy},
  year={2005},
  volume={220 Pt 3},
  pages={176-89}
}
Micrographs are two-dimensional (2D) representations of three-dimensional (3D) objects. When the depth-of-field of a micrograph is comparable with or larger than the characteristic dimension of objects within the micrograph, measured 2D parameters (e.g. particle number density, surface area of particles, fraction of open space) require stereological correction to determine the correct 3D values. Here, we develop a stereological theory using a differential approach to relate the 3D volume… CONTINUE READING

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