Studies on chymotrypsin-like catalysis by synthetic peptides

@article{Corey1994StudiesOC,
  title={Studies on chymotrypsin-like catalysis by synthetic peptides},
  author={Michael J. Corey and Eva Hallakova and Kathleen C. Pugh and John M Stewart},
  journal={Applied Biochemistry and Biotechnology},
  year={1994},
  volume={47},
  pages={199-212}
}
The synthetic peptide Chymohelizyme-1 (CHZ-1) exhibits esterase activity against carbobenzoxytyrosinep-nitrophenyl ester (ZTONP), carbobenzoxyalaninep-nitrophenyl ester (ZAONP), andt-butyloxycarbonyltyrosinep-nitrophenyl ester (BocTONP). However, earlier reports of catalytic activity against less labile esters and amides have proven to be incorrect. The major reason for the errors appears to have been the omission of certain controls in the previous work. Although the catalytic triad does not… 
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14 Citations
Background Citations
1
Methods Citations
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14 Citations

Design, synthesis and catalytic activity of a serine protease synthetic model
TLDR
Results suggest that the designed carrier brings in appropriate contact the catalytic triad residues (Ser, His, Asp) resulting in the obtained hydrolytic activity.
De novo designed polypeptide catalysts with adopted folded structures
TLDR
It was found that so far the most severe limitation of folded polypeptide catalysts is the efficiency obtained in the bond-making and bond-breaking steps, whereas the binding of substrates, even on the surface of helical structures in aqueous solution, is of comparable strength to that which occurs in nature.
Polypeptides with supersecondary structures as templates in rational catalyst design. Catalysis of self functionalization by designed helix–loop–helix motifs
TLDR
The ability of a designed helix–loop–helix motif, RA-42, to catalyse its own functionalization is thereby demonstrated for the first time.
Interhelical carbon bridging of catalytic helix–loop–helix polypeptide motifs
TLDR
The catalysis of the site-selective reaction has been investigated in detail using a number of designed helix–loop–helix polypeptide motifs that have been reacted with DSG and the longer N-hydroxysuccinimide ester bis(sulfosucc inimidyl) suberate (BS3).
Structure of a cyclic peptide with a catalytic triad, determined by computer simulation and NMR spectroscopy
TLDR
A cyclic, eight-residue peptide that possesses the catalytic triad residues of the serine proteases is reported that is synthesised and purified and hydrolyses p-nitrophenyl acetate about nine times faster than free histidine.
Site-selective control of the reactivity of surface-exposed histidine residues in designed four-helix-bundle catalysts.
Synthesis of functionalised nucleosides for incorporation into nucleic acid-based serine protease mimics.
TLDR
The synthesis of nucleosides modified with an extra imidazole, carboxyl and hydroxyl group can be incorporated into an oligonucleotide duplex, thus generating a novel type of serine protease mimic.
Functionalization and Properties of Designed Folded Polypeptides
TLDR
Strategies for functionalization using both the naturally occurring amino acids and post-synthetic incorporation of non-natural functionality will be described, as well as the level of function that has been achieved by rational design.
De Novo Design of Protein Structure and Function
Protein Design: A Hierarchic Approach
The de novo design of peptides and proteins has recently emerged as an approach for investigating protein structure and function. Designed, helical peptides provide model systems for dissecting and…
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References

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