Studies on chymotrypsin-like catalysis by synthetic peptides

  title={Studies on chymotrypsin-like catalysis by synthetic peptides},
  author={Michael J. Corey and Eva Hallakova and Kathleen C. Pugh and John M Stewart},
  journal={Applied Biochemistry and Biotechnology},
The synthetic peptide Chymohelizyme-1 (CHZ-1) exhibits esterase activity against carbobenzoxytyrosinep-nitrophenyl ester (ZTONP), carbobenzoxyalaninep-nitrophenyl ester (ZAONP), andt-butyloxycarbonyltyrosinep-nitrophenyl ester (BocTONP). However, earlier reports of catalytic activity against less labile esters and amides have proven to be incorrect. The major reason for the errors appears to have been the omission of certain controls in the previous work. Although the catalytic triad does not… 
Design, synthesis and catalytic activity of a serine protease synthetic model
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De novo designed polypeptide catalysts with adopted folded structures
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Polypeptides with supersecondary structures as templates in rational catalyst design. Catalysis of self functionalization by designed helix–loop–helix motifs
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A cyclic, eight-residue peptide that possesses the catalytic triad residues of the serine proteases is reported that is synthesised and purified and hydrolyses p-nitrophenyl acetate about nine times faster than free histidine.
Synthesis of functionalised nucleosides for incorporation into nucleic acid-based serine protease mimics.
The synthesis of nucleosides modified with an extra imidazole, carboxyl and hydroxyl group can be incorporated into an oligonucleotide duplex, thus generating a novel type of serine protease mimic.
Functionalization and Properties of Designed Folded Polypeptides
Strategies for functionalization using both the naturally occurring amino acids and post-synthetic incorporation of non-natural functionality will be described, as well as the level of function that has been achieved by rational design.
Protein Design: A Hierarchic Approach
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Design and synthesis of a peptide having chymotrypsin-like esterase activity.
A peptide having enzyme-like catalytic activity has been designed and synthesized and is inhibited by ChTr specific inhibitors and is inactive toward benzoyl arginine ethyl ester, a trypsin substrate.
Synthesis and stability of 3-nitro-2-pyridinesulfenyl chloride (NpysCl).
The stability of NpysCl was determined in a variety of solvents, with and without base, to determine the most suitable solvent and base for the synthesis of N-Npys amino acids.
The binding of nucleotides by pancreatic ribonuclease. I. Proton uptake and release associated with anion binding.
Interactions involving both electrostatic and hydrogen bonding between the phosphate group and the binding site appear to explain the considerable stability of RNase-nucleotide complexes, and changes in the H+ concentration caused by the addition of various competitive inhibitors at the same pH.
Solid Phase Peptide Synthesis
This work has shown that effective methods of peptide synthesis are crucial to progress in this area, because only by synthesis can adequate amounts of important peptides be made available for chemical, biologi...
Effects of resin swelling and substitution on solid phase synthesis.
Effects of resin swelling, uniformity, and substitution on the solid phase synthesis of long, structured and/or branched peptides were evaluated.