Studies on carbohydrate moieties ofAspergillus niger glucoamylase II: Isolation, purification and characterization of glycopeptides

Six glycopeptide fractions namely GP-C1, GP-C2. GP-C3a.GP-C3b.GP-D, and GP-D2 were isolated after exhaustive digestion of glucoamylase II (Glucozyme) fromAspergillus niger with pronase. They were purified using gel-filtration. high-voltage paper electrophoresis and ion-exchange chromatography on Dowex-50 and Dowex-1. They appeared homogeneous on… (More)