Studies on a new immunoactive peptide, FK-156. II. Fermentation, extraction and chemical and biological characterization.

  title={Studies on a new immunoactive peptide, FK-156. II. Fermentation, extraction and chemical and biological characterization.},
  author={T. Gotoh and K. Nakahara and T. Nishiura and M. Hashimoto and T. Kino and Y. Kuroda and M. Okuhara and M. Kohsaka and H. Aoki and H. Imanaka},
  journal={The Journal of Antibiotics},
: An interesting immunoactive peptide, FK-156, has been isolated from the fermentation broth of Streptomyces olivaceogriseus sp. nov. and Streptomyces violaceus. The compound was purified by column chromatography with activated carbon, ion exchange Sephadex and cellulose powder. FK-156, obtained as white powder, exhibits a wide variety of immunostimulatory activity in vivo and in vitro with experimental animals. Pretreatment of mice or rats with this peptide protected the animals against lethal… Expand
Studies on a new immunoactive peptide, FK-156. I. Taxonomy of the producing strains.
Two strains of actinomycetes produce a new biologically active substance, designated FK-156, and are identified as Streptomyces olivaceogriseus sp. Expand
Selective suppression by prodigiosin of the mitogenic response of murine splenocytes.
Prodigiosin 25-C completely inhibited induction of cytotoxic T cells in a mixed lymphocyte reaction (MLR) at 4 ng/ml. Expand
Immunoactive peptides, FK-156 and FK-565. I. Enhancement of host resistance to microbial infection in mice.
After subcutaneous dosing with FK-156, the enhancement of host defense of mice against E. coli infection was more rapid than against Listeria infection, and the enhancing effects of Fk-156 and FK -565 on host defenseof mice against pseudomonal infections was more potent than other immunoactive drugs. Expand
Immunoactive peptides, FK 156 and FK 565. IV. Activation of mouse macrophages.
Both drugs directly enhanced the production of superoxide anion by mouse peritoneal macrophages treated in vitro and enhanced the functions of peritoneals macrophage of athymic nude mice, suggesting that direct activation might be one of the mechanisms of Macrophage activation by the peptides. Expand
Biologically active secondary metabolites from Streptomyces
Abstract An extensive research program for isolation and studies on the mechanism of action of secondary metabolites has documented a wider group of compounds that are not antimicrobial agents. AExpand
Aladapcin, a new microbial metabolite that enhances host resistance against bacterial infection. Production, isolation, physico-chemical properties and biological activities.
Aladapcin enhanced host resistance against an experimental Escherichia coli infection in mice at doses ranging between 1 and 100 micrograms/kg. Expand
Immunomodulatory and therapeutic properties of FK-565 in mice
FK-565 had significant immunoaugmentary properties for natural killer cells, macrophages, and T cells both in vitro and in vivo, suggesting that its therapeutic activity is due to immune augmentation. Expand
Immunomodulators isolated from microorganisms
Microbial products are surveyed that have an immunoregulatory activity, both from the realm of low-molar-mass compounds and from the group of naturally occurring polymers. The data include in mostExpand
T-cell-dependent immunobiological activity of a desmuramyl analog of muramyl dipeptide, adamantylamide dipeptide (AdDP), and D-isoglutamine.
The present experiments demonstrate that, similar to immunomodulatory muramyl dipeptide, its desmuramyl analog adamantylamide dipeptide is able to induce mild and fully reversible paw edema in mice.Expand
In vitro effects of an acyltripeptide, FK565, on antitumor effector activities and on metabolic activities of human monocytes and granulocytes.
Data indicate that in vitro incubation of human monocytes and granulocytes with FK565 (0.1-100 micrograms/ml) had resulted in simultaneous up-regulation of several anti-tumor functions mediated by these cells. Expand