Studies on a Doubleheaded Protease Inhibitor from Phaseolus mungo

  title={Studies on a Doubleheaded Protease Inhibitor from Phaseolus mungo},
  author={N. Hajela and Abhay H Pande and Sandeep Sharma and Desirazu Narasimha Rao and Krishnan Hajela},
  journal={Journal of Plant Biochemistry and Biotechnology},
A doubleheaded protease inhibitor showing inhibition of bovine pancreatic trypsin and α-chymotrypsin was isolated and purified from the seeds of Phaseolus mungo. The molecular weight of the protease inhibitor was found to be 14.2 kD by SDS-PAGE analysis and gel filtration. The native inhibitor inhibited trypsin and α-chymotrypsin stoichiometrically at the molar ratio 1:1 and 2:1 respectively. The Ki app for trypsin was found to be 0.35 nM and for α-chymotrypsin to be 2.4 nM. Bovine pepsin was… CONTINUE READING
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