Studies on Xenopus immunoglobulins using monoclonal antibodies.

  title={Studies on Xenopus immunoglobulins using monoclonal antibodies.},
  author={Ellen Hsu and Louis du Pasquier},
  journal={Molecular immunology},
  volume={21 4},
Monoclonal antibodies raised against Xenopus Ig recognize antigenic determinants on IgM and low mol. wt Ig (LMW Ig or IgY ). On SDS-PAGE two forms of mu were distinguished in the supernatant and cell lysate of tunicamycin-treated spleen cell culture. The two bands of v observed in serum appear to be the result of carbohydrate heterogeneity. The light chains resolved into two distinct bands which differed in peptide maps; the more slowly migrating band of light chain was preferentially… CONTINUE READING

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