Studies on 5 s RNA conformation by partial ribonuclease hydrolysis.

@article{Jordan1971StudiesO5,
  title={Studies on 5 s RNA conformation by partial ribonuclease hydrolysis.},
  author={Bertrand R. Jordan},
  journal={Journal of molecular biology},
  year={1971},
  volume={55 3},
  pages={
          423-39
        }
}
Abstract 32 P-labeled native 5 s RNA from Escherichia coli was digested under mild conditions with T 1 or pancreatic RNases; the hydrolysate was analysed on an acrylamide gel slab in non-denaturing conditions and was resolved into a small number of discrete bands, which were eluted and characterized by fingerprinting. Definite sequences can be assigned to the corresponding fragments; exposed, singlestranded regions can be defined and self-paired nucleotide stretches are identified. 8 m -urea… CONTINUE READING