Studies on (−)-cathinone formation in micropropagated plants and tissue cultures of Catha edulis (Khat)

  title={Studies on (−)-cathinone formation in micropropagated plants and tissue cultures of Catha edulis (Khat)},
  author={M. M. El-domiaty and H. M. Elhag and Farouk S. El-Feraly and I. A. Al-meshal and Mahmoud M. El-Olemy},
AbstractA highly sensitive HPLC method (minimum detection limit 150 ng) is reported for the determination of (-)-cathinone in micropropagated plants and in tissue cultures of Catha edulis (khat). Callus was initiated from leaves or shoot tips of khat on MS media containing either IB A (indolebutyric acid) or NAA (naphthalene acetic acid). Callus growth was best obtained in the light on media containing 3.0 mg/1 IBA or NAA. The inclusion of 1.0 g/1 of PVP in the medium was found necessary to… Expand
Antimicrobial and Cytotoxic Activity of the Extracts of Khat Callus Cultures
In the course of the work with khat tissue cultures, the production of dark colored pigments was observed as a typical characteristic of the callus culture, and the present investigation deals with the isolation, identification, and biological effects of such pigments. Expand
Catha edulis (Khat): In Vitro Culture and the Production of Cathinone and Other Secondary Metabolites
Khat, Catha edulis (Vahl) Forssk. ex Endl. (Celasteraceae), is an evergreen shrub or tall tree that may reach up to 25 m in height if not pruned (Fig. 1). Extensive pruning makes it a small shrub, asExpand
Enhanced HIV‐1 Reverse Transcriptase Inhibitory and Antibacterial Properties in Callus of Catha edulis Forsk.
Tissue culture‐derived calli could be used as therapeutic agents for traditional medicine and the choice of treatment used in the tissue culture system and the age of the callus for production of biomass may significantly influence its therapeutic potential. Expand
Volatile Constituents of the Leaves of Mikania cordata (Burm.f.) B.L. Robinson var. cordata (Asteraceae)
The leaf oil and extract of Mikania cordata from Cote d'Ivoire was found to contain β-caryophyllene and germacrene D as major components, respectively. Expand


In Vitro Propagation of Catha edulis
In vitro propagation of khat is described to provide reliable and uniform plant material for further studies on secondary plant products and to investigate its pharmacological properties. Expand
Selection and micropropagation of high artemisinin producing clones of Artemisia annua L.
Results from unorganized callus cultures initiated from the leaves of the corresponding clones did not indicate the formation of artemisinin or related sesquiterpenes in these cultures. Expand
The chemistry of khat.
  • K. Szendrei
  • Chemistry, Medicine
  • Bulletin on narcotics
  • 1980
Cathinone is the main phenylalkylamine component of fresh khat, and pharmacological studies indicate that it may be the compound responsible for the characteristic stimulant activity and abuse potential of the plant. Expand
Nutrient requirements of suspension cultures of soybean root cells.
Abstract The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyaceticExpand
A revised medium for rapid growth and bio assays with tobacco tissue cultures
In experiments with tobacco tissue cultured on White's modified medium supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Expand
Plant tissue and cell culture
Plant tissue and cell culture is a process that involves cloning plants/plant cells from individual cells, organs or tissue in a nutrient medium under sterile conditions and forms an essential component of scientific research on plants. Expand
The morphology and anatomy of the leaves of Catha edulis Forsk. obtained from Ethiopia is described and illustrated. A list of the characters of powdered Khat is also given.
Pharmacological properties of the stimulant khat.
  • P. Kalix
  • Medicine
  • Pharmacology & therapeutics
  • 1990
The pharmacology of khat and of its active constituents is summarized to describe briefly the khat habit and to summarize the pharmacological basis for the effects of this drug. Expand