Studies of tobacco mosaic virus reassembly with an RNA tail blocked by a hybridised and cross-linked probe.

@article{Fairall1986StudiesOT,
  title={Studies of tobacco mosaic virus reassembly with an RNA tail blocked by a hybridised and cross-linked probe.},
  author={Louise Fairall and John T. Finch and Celine Fan Fong Hui and Charles R. Cantor and P. Jonathan G. Butler},
  journal={European journal of biochemistry},
  year={1986},
  volume={156 3},
  pages={459-65}
}
Segments of cloned cDNA to tobacco mosaic virus RNA, 150--300-bases long, have been hybridised and cross-linked to the RNA, which has then been used for reassembly experiments. This enables the elongation reaction, which does not encapsidate the double-stranded region generated, to be stopped at specific regions along the RNA and the resulting particles to be characterised, by measuring the lengths of the rods in the electron microscope. With hybridisation to the 3'-tail the entire RNA… CONTINUE READING