Studies of the oxidative half-reaction of anthranilate hydroxylase (deaminating) with native and modified substrates.


The oxidative half-reactions of anthranilate hydroxylase (EC were examined in the presence of anthranilate and modified substrates. C(4a)-Hydroperoxyflavin (C(4a)-FlOOH) and C(4a)-hydroxyflavin (C(4a)-FlOH) intermediates were detected in oxidative reactions with all substrates. Thus, the oxygenation reactions of the enzyme are similar to those of… (More)