Studies of the metabolic N- and O-demethylation of [6-3H]codeine.

  title={Studies of the metabolic N- and O-demethylation of [6-3H]codeine.},
  author={P H Duquette and Frank Peterson and Daune L Crankshaw and Nico Lindemann and Jordan L. Holtzman},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  volume={11 5},
We have modified our radiometric assay for ethylmorphine N-demethylase to examine the metabolism of codeine. We find that the current assay gives excellent separation of metabolites with a zero time activity of 0.08%. The N- and O-demethylations are linear for up to 30 min with up to 1 mg/ml of microsomal protein. The pH profiles show slightly different… CONTINUE READING