Studies of the blood ofAscidia ceratodes. Total blood cell counts, differential blood cell counts, hematocrit values, seasonal variations, and fluorescent characteristics of blood cells

  title={Studies of the blood ofAscidia ceratodes. Total blood cell counts, differential blood cell counts, hematocrit values, seasonal variations, and fluorescent characteristics of blood cells},
  author={Wilton R. Biggs and James H. Swinehart},
Seasonal, and animal size and weight variations of the blood cells of the vanadium-containing ascidian,Ascidia ceratodes, were determined. The fluorescent properties of various cell types were ascertained, and discussed in terms of cell development, phylogenic position of the species, and chemicals in the cells. 
4 Citations
The ecophysiology of iron and vanadium accumulation by North Atlantic ascidians
This thesis examines the seasonal variation in vanadium and iron concentrations of two Phlebobranch ascidians, Ciona intestinalis and Ascidia callosa, which accumulate vananium and iron, and a Stolidobranch Ascidian, Halocynthia pyriformis, which accumulates iron only.
Vanadium biochemistry: The unknown role of vanadium-containing cells in ascidians (sea squirts)
A hypothesis correlating ascidian blood cell function to anaerobiosis, via two prominent redox constituents, namely vanadium(III) and the tunichromes, a family of metal ion complexing/reducing hydroquinonoid peptides, is presented.


The blood of Ascidia nigra: blood cell frequency distribution, morphology, and the distribution and valence of vanadium in living blood cells.
Density separation of blood cells has been achieved; this result coupled with various cell morphologies suggests that different cells may represent different maturational stages in a developmental process.
The histology of the blood of some bermuda ascidians
The structure of the living blood cells is described and the cells are classified in the following species of ascidians: Phallusia nigra, Ecteinascidia turbinata, Clavelina oblongata, Symplegma
Observations on the blood of Certain Ascidians, with Special Reference to the Biochemistry of Vanadium
Experimental evidence and theoretical considerations are brought forward to show that the vanadium chromogen is not in any sense a respiratory pigment, and it seems possible that sea water provides a sufficiently rich source of vanadium to account for the amounts absorbed.
The vanadium and selected metal contents of some ascidians.
The vanadium and other selected metal contents of primarily California ascidians have been determined and three dominant fractions were chromatographed from the cells contained in the fluid of Ascidia ceratodes.
Fluorescence of blood cells of the tunicatesPhallusia mamillata andCiona intestinalis
Von den Blutzellen derPhallusia mamillata Cuvier zeigen nur die stark vakuolisierten (compartment cells) im UV-Licht eine stabile blaue Fluoreszenz (λ max =5200 and 4600 Å) auf, die nur von den Vanadocyten ausgeht.
Nuclear magnetic resonance spectrum of living tunicate blood cells and the structure of the native vanadium chromogen.
  • R. Carlson
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1975
The 1-H nuclear magnetic resonance spectrum of living tunicate blood cells was examined in an attempt to develop a biophysical assay for the native vanadium chromogen and revealed that vanadium(III) concentrations are rigidly regulated within these vacuoles, and that artifact formation does occur in the hemolysate.
Über ein Vorkommen von Purinen und eines Pterins in einer Ascidienart (Microcosmus polymorphus)
Aus den inneren Organen einer Ascidienart (Microcosmus polyxnorphus) wurden Harnsaure, 2-Amino-6,8-dioxy-purin und Xanthopterin isoliert.
Computer-aided biochemical system analysis in open systems with environment simulation
A computer-aided arrangement is described which allows kinetic and regulative studies with enzymes, organelles and cells in an open system. This is demonstrated with some simple examples.