Studies of the Escherichia coli Rsd-sigma70 complex.

  title={Studies of the Escherichia coli Rsd-sigma70 complex.},
  author={Lars F. Westblade and Leopold L Ilag and Andrew Powell and Annie Kolb and Carol V Robinson and Stephen J W Busby},
  journal={Journal of molecular biology},
  volume={335 3},
Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex. 

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