Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.

@article{Kadesch1982StudiesOI,
  title={Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.},
  author={Tom R. Kadesch and Michael J. Chamberlin},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 9},
  pages={5286-95}
}
Addition of 10 to 100 oligodeoxycytidylate residues to the 3'-OH termini of T7 bacteriophage DNA produces a highly efficient template for transcription in vitro with purified calf thymus RNA polymerase II. Transcription initiates rapidly and selectively at the oligo (dC) ends of such a template and essentially all of the active RNA polymerase II molecules are then committed to a long period of RNA chain elongation. This allows the direct study of the RNA chain elongation and termination… CONTINUE READING