Studies of binding of tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) to fibroblast growth factor inducible 14 (Fn14).

@article{Fick2012StudiesOB,
  title={Studies of binding of tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) to fibroblast growth factor inducible 14 (Fn14).},
  author={Andrea Fick and Isabell Lang and Viktoria Sch{\"a}fer and Axel Seher and Johannes Trebing and Daniel J. Weisenberger and Harald Wajant},
  journal={The Journal of biological chemistry},
  year={2012},
  volume={287 1},
  pages={
          484-95
        }
}
To perform highly sensitive cellular binding studies with TNF-like weak inducer of apoptosis (TWEAK), we developed a bioluminescent variant of soluble TWEAK (GpL-FLAG-TNC-TWEAK) by fusing it genetically to the C terminus of the luciferase of Gaussia princeps (GpL). Equilibrium binding studies on human (HT1080 and HT29) and murine (Renca and B16) cell lines at 37 °C revealed high affinities of human TWEAK from 53 to 112 pm. The dissociation rate constant of the TWEAK-Fn14 interaction was between… CONTINUE READING
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References

Publications referenced by this paper.
SHOWING 1-4 OF 4 REFERENCES

NUMBER 1 JOURNAL OF BIOLOGICAL CHEMISTRY 495 at U niersity of M icigan on M arch 3, 2015 hp://w w w .jb.org/ D

R. Feltham, M. Moulin, +6 authors J. Silke
  • J. Biol. Chem
  • 2010

NUMBER 1 JOURNAL OF BIOLOGICAL CHEMISTRY 495 at U niersity of M icigan on M arch 3, 2015 hp://w w w .jb.org/ D

R. Feltham, M. Moulin, +6 authors J. Silke
  • J. Biol. Chem
  • 2010

TWEAK-Fn14 Binding Studies 494 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME

S. Desplat-Jégo, S. Varriale, +6 authors J. Boucraut
  • J. Neuroimmunol. 133,
  • 2002

TWEAK-Fn14 Binding Studies 494 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME

S. Desplat-Jégo, S. Varriale, +6 authors J. Boucraut
  • J. Neuroimmunol. 133,
  • 2002