Studies in the development of Japanese encephalitis vaccine: expression of virus envelope glycoprotein V3 (E) gene in yeast.

@article{Fujita1987StudiesIT,
  title={Studies in the development of Japanese encephalitis vaccine: expression of virus envelope glycoprotein V3 (E) gene in yeast.},
  author={Hiroya Fujita and Hideaki Sumiyoshi and Chisato Mori and Susumu Manabe and Masatoshi Takagi and Ichiro Yoshida and Koji Morita and Isao Fuke and Katsuhiko Fukai and Akira Igarashi},
  journal={Bulletin of the World Health Organization},
  year={1987},
  volume={65 3},
  pages={303-8}
}
A safe, effective and economical vaccine is required for the prevention of Japanese encephalitis (JE), a disease with high mortality and grave sequelae, which is prevalent in Japan and other countries in east, south-east and southern Asia. As the initial step to produce a second-generation vaccine, recombinant DNA technology was utilized to express the JE virus envelope glycoprotein V3 (E) gene in yeast cells.This report describes the construction of a yeast expression vector in which a cDNA… CONTINUE READING