Studies in red blood cell preservation. 6. Red cell membrane remodeling during rejuvenation.

@article{Dumaswala1992StudiesIR,
  title={Studies in red blood cell preservation. 6. Red cell membrane remodeling during rejuvenation.},
  author={U J Dumaswala and T L Petrosky and Tibor J. Greenwalt},
  journal={Vox sanguinis},
  year={1992},
  volume={63 1},
  pages={12-5}
}
The purpose of this study was to examine whether vesiculation of RBC plays a significant role in their rejuvenation. Outdated units of Adsol blood, were divided into two aliquots and incubated with equal volumes of a solution of 100 mM pyruvate and inosine, 103 mM phosphate and 5 mM adenine (PIPA) or 0.9% saline. Following 1 h incubation, vesicles were isolated from the supernatants and quantitated for hemoglobin content. Restoration of RBC ATP, 2,3-DPG, morphology, and osmotic fragility after… CONTINUE READING