Stu1 inversely regulates kinetochore capture and spindle stability.

  title={Stu1 inversely regulates kinetochore capture and spindle stability.},
  author={Jennifer Ort{\'i}z and Caroline Funk and Astrid Sch{\"a}fer and Johannes Lechner},
  journal={Genes & development},
  volume={23 23},
The Saccharomyces cerevisiae CLASP (CLIP-associated protein) Stu1 is essential for the establishment and maintenance of the mitotic spindle. Furthermore, Stu1 localizes to kinetochores. Here we show that, in prometaphase, Stu1 assembles in an Ndc80-dependent manner exclusively at kinetochores that are not attached to microtubules. Stu1 relocates to microtubules when a captured kinetochore reaches a spindle pole. This relocation does not depend on kinetochore biorientation, but requires a… CONTINUE READING