Structures of dNTP intermediate states during DNA polymerase active site assembly.

@article{Freudenthal2012StructuresOD,
  title={Structures of dNTP intermediate states during DNA polymerase active site assembly.},
  author={Bret D. Freudenthal and William A. Beard and Samuel H. Wilson},
  journal={Structure},
  year={2012},
  volume={20 11},
  pages={1829-37}
}
DNA polymerase and substrate conformational changes are essential for high-fidelity DNA synthesis. Structures of DNA polymerase (pol) β in complex with DNA show the enzyme in an "open" conformation. Subsequent to binding the nucleotide, the polymerase "closes" around the nascent base pair with two metals positioned for chemistry. However, structures of… CONTINUE READING