Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.

@article{Ducka2010StructuresOA,
  title={Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.},
  author={Anna M Ducka and Peteranne B. Joel and G M Popowicz and Kathleen M Trybus and Michael Schleicher and Angelika A. Noegel and Robert Huber and Tad A. Holak and Tomasz Sitar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 26},
  pages={11757-62}
}
Three classes of proteins are known to nucleate new filaments: the Arp2/3 complex, formins, and the third group of proteins that contain ca. 25 amino acid long actin-binding Wiskott-Aldrich syndrome protein homology 2 domains, called the WH2 repeats. Crystal structures of the complexes between the actin-binding WH2 repeats of the Spire protein and actin were determined for the Spire single WH2 domain D, the double (SpirCD), triple (SpirBCD), quadruple (SpirABCD) domains, and an artificial Spire… CONTINUE READING