Structures of Saccharomyces cerevisiae d-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition


The primary role of yeast Ara1, previously mis-annotated as a D-arabinose dehydrogenase, is to catalyze the reduction of a variety of toxic α,β-dicarbonyl compounds using NADPH as a cofactor at physiological pH levels. Here, crystal structures of Ara1 in apo and NADPH-complexed forms are presented at 2.10 and 2.00 Å resolution, respectively. Ara1 exists as… (More)
DOI: 10.1107/S1744309113026857


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