Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells.

  title={Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells.},
  author={Nirianne M Q Palacpac and Yoshinobu Kimura and Kazuhito Fujiyama and Tadashi Yoshida and Tomoaki Seki},
  journal={Bioscience, biotechnology, and biochemistry},
  volume={63 1},
The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major… CONTINUE READING

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