Structures of HIV-1 RT–DNA complexes before and after incorporation of the anti-AIDS drug tenofovir

  title={Structures of HIV-1 RT–DNA complexes before and after incorporation of the anti-AIDS drug tenofovir},
  author={Steve Tuske and Stefan G. Sarafianos and Arthur D. Clark and Jianping Ding and Lisa K Naeger and Kirsten Lofgren White and Michael D. Miller and Craig S Gibbs and Paul L. Boyer and Patrick Clark and Gang Wang and Barbara L. Gaffney and Roger A. Jones and Donald M. Jerina and Stephen H. Hughes and Eddy Arnold},
  journal={Nature Structural &Molecular Biology},
Tenofovir, also known as PMPA, R-9-(2-(phosphonomethoxypropyl)adenine, is a nucleotide reverse transcriptase (RT) inhibitor. We have determined the crystal structures of two related complexes of HIV-1 RT with template primer and tenofovir: (i) a ternary complex at a resolution of 3.0 Å of RT crosslinked to a dideoxy-terminated DNA with tenofovir-diphosphate bound as the incoming substrate; and (ii) a RT–DNA complex at a resolution of 3.1 Å with tenofovir at the 3′ primer terminus. The tenofovir… CONTINUE READING


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